Streptomyces coelicolor DNA homologous with acyltransferase domains of type I polyketide synthase gene complex
نویسندگان
چکیده
منابع مشابه
A Streptomyces coelicolor host for the heterologous expression of Type III polyketide synthase genes
BACKGROUND Recent advances in genome sequencing, combined with bioinformatic analysis, has led to the identification of numerous novel natural product gene clusters, particularly in actinomycetes of terrestrial and marine origin. Many of these gene clusters encode uncharacterised Type III polyketide synthases. To facilitate the study of these genes and their potentially novel products, we set o...
متن کاملType I polyketide synthase requiring a discrete acyltransferase for polyketide biosynthesis.
Type I polyketide synthases (PKSs) are multifunctional enzymes that are organized into modules, each of which minimally contains a beta-ketoacyl synthase, an acyltransferase (AT), and an acyl carrier protein. Here we report that the leinamycin (LNM) biosynthetic gene cluster from Streptomyces atroolivaceus S-140 consists of two PKS genes, lnmI and lnmJ, that encode six PKS modules, none of whic...
متن کاملExpression of a functional fungal polyketide synthase in the bacterium Streptomyces coelicolor A3(2).
The multifunctional 6-methylsalicylic acid synthase gene from Penicillium patulum was engineered for regulated expression in Streptomyces coelicolor. Production of significant amounts of 6-methylsalicylic acid by the recombinant strain was proven by nuclear magnetic resonance spectroscopy. These results suggest that it is possible to harness the molecular diversity of eukaryotic polyketide path...
متن کاملType II thioesterase ScoT is required for coelimycin production by the modular polyketide synthase Cpk of Streptomyces coelicolor A3(2).
Type II thioesterases were shown to maintain efficiency of modular type I polyketide synthases and nonribosomal peptide synthetases by removing acyl residues blocking extension modules. We found that thioesterase ScoT from Streptomyces coelicolor A3(2) is required for the production of the yellow-pigmented coelimycin by the modular polyketide synthase Cpk. No production of coelimycin was observ...
متن کاملGene recombination in Streptomyces coelicolor.
Prototrophic recombinant strains were obtained by plating on minimal medium spores from mixed cultures grown on limiting agar, of two strains each with a different double nutritional requirement. Clones showing new associations of properties were isolated on partially supplemented media. Some showed one ‘ mutant ’ character derived from one of the starting strains and ‘wild’ characters derived ...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1997
ISSN: 0378-1097
DOI: 10.1016/s0378-1097(97)00476-x